Blotto Beta
Immunoblot (Western blot) quantification

See the number —
and everything behind it.

Blotto™ shows your immunoblot, the bands it measured, and the value it got — all on one screen. Trust the number because you can see exactly how it was made.

Be a Beta Tester for Blotto See how it works

In your browser · nothing to install · no data stored

Blotto in action: the annotated blot, the quantification chart, and the 3-D signal terrain — all in one view
1The outline is the number.
The band's real footprint — every pixel shown.
2A number you can defend.
Signal above the noise, with its own ±.
3Your signal, as a landscape.
Bands rise from a flat noise floor.

Densitometry is quietly broken.

The trustworthy tools are expensive and locked to one instrument. Everyone else eyeballs band intensity or wrestles ImageJ into giving a number — hand-tuned, unrecorded, and different from one person, day, or figure to the next. Two trainees measure the same immunoblot and disagree.

Blotto fixes that — accessible, consistent, transparent.

Numbers you can defend

It catches what quietly ruins immunoblot data.

The measurement line sits a set margin above the image's own noise — the same rule on every band, strong or faint. And when a band hits the sensor ceiling, Blotto flags it and shows the saturation plane in 3-D, so a clipped value never sneaks into your figure.

You can't hand-edit a value — you only choose where to measure. That's what makes it reproducible.

A saturation warning and the 3-D view showing the sensor-ceiling plane cutting through clipped bands
No setup · no downloads · no vendor software

Point it at your export folder.

Blotto reads Bio-Rad ChemiDoc, Invitrogen iBright, and LI-COR Odyssey — one workflow, any imager. Drop in a folder and it detects the channels, orders the exposures, picks the measurement-grade files, and sets aside the display exports and junk — and tells you what it skipped.

Everything runs in your browser. Your image files are never uploaded — only the region you select is sent for analysis, and nothing is stored.

Blotto detecting two channels and their exposures from a Bio-Rad export folder
Multi-exposure, done honestly

The whole picture, in one view.

Don't cherry-pick an exposure. Blotto takes in every frame and shows the entire analysis on one screen.

The blot, the per-band volumes, the 3-D surface, the exposure-series linearity, and the pooled relative amount with its confidence interval — together, nothing hidden in another tab. The reliable exposures pool into one number you can defend.

One quantification channel with every part of the analysis in a single view — the blot, per-band volumes, 3-D surface, exposure-series charts, and the pooled relative amount
Normalize the way reviewers expect

Loading control or total protein — built in.

Normalize your target to a loading-control channel or to whole-lane total protein, per lane, right in the workflow — not in a spreadsheet afterward. The reference and the ratio are visible, not hidden.

Two fluorescence channels with per-lane normalization to a loading control
Close, stacked bands

It finds where to split bands in a crowded lane.

Two bands stacked in one lane? Where one ends and the next begins is exactly where a box — or a straight line — gets it wrong.

Blotto reads the signal and draws the divider along the valley between them — the real boundary, curved to follow the gel — so each band is targeted on its own. You see the split it chose, right on the image, and can nudge it before you quantify.

Two bands stacked in each lane, separated by a curved divider that follows the valley between them
How it works

Four steps, start to figure.

1

Load your immunoblot

Any imager, any common format. It opens in your browser — nothing is uploaded.

2

Point at what matters

Mark the region, confirm the lanes, click the bands. (Load your immunoblot in the standard orientation.)

3

Quantify

Blotto measures each band's real footprint above the noise — and shows the pixels it counted.

4

Export with provenance

Your numbers plus a record of how they were made — ready for the figure and the methods.

See exactly what you're measuring.

Reproducible immunoblot numbers you can put in a paper and defend to a reviewer.

Runs in your browser · nothing to install · your images never leave your machine

Good to know

iIndependent — no vendor affiliation

Blotto is an independent product, not affiliated with, endorsed by, or sponsored by any imaging-system vendor. Bio-Rad, ChemiDoc, Image Lab, Invitrogen, iBright, LI-COR, Odyssey, and other product names are trademarks of their respective owners, referenced only to describe file-format compatibility.

iYour data stays yours

Blotto runs in your browser, and your image files are never uploaded. Only the unlabeled region of interest you select — with no sample names, filenames, or identifying metadata — is sent to the Blotto server to run the analysis. Nothing is stored on our servers.